Refinement for single-nanoparticle structure determination from low-quality single-shot coherent diffraction data - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Accéder directement au contenu
Article Dans Une Revue International Union of Crystallography journal Année : 2020

Refinement for single-nanoparticle structure determination from low-quality single-shot coherent diffraction data

Yuhiro Sato
  • Fonction : Auteur
Tsukasa Sakai
  • Fonction : Auteur
Kenji Matsunami
  • Fonction : Auteur

Résumé

With the emergence of X-ray free-electron lasers, it is possible to investigate the structure of nanoscale samples by employing coherent diffractive imaging in the X-ray spectral regime. In this work, we developed a refinement method for structure reconstruction applicable to low-quality coherent diffraction data. The method is based on the gradient search method and considers the missing region of a diffraction pattern and the small number of detected photons. We introduced an initial estimate of the structure in the method to improve the convergence. The present method is applied to an experimental diffraction pattern of an Xe cluster obtained in an X-ray scattering experiment at the SPring-8 Angstrom Compact free-electron LAser (SACLA) facility. It is found that the electron density is successfully reconstructed from the diffraction pattern with a large missing region, with a good initial estimate of the structure. The diffraction pattern calculated from the reconstructed electron density reproduced the observed diffraction pattern well, including the characteristic intensity modulation in each ring. Our refinement method enables structure reconstruction from diffraction patterns under difficulties such as missing areas and low diffraction intensity, and it is potentially applicable to the structure determination of samples that have low scattering power.
Fichier principal
Vignette du fichier
cw5023.pdf (1.31 Mo) Télécharger le fichier
Origine Publication financée par une institution
Licence

Dates et versions

cea-04115269 , version 1 (02-06-2023)

Licence

Identifiants

Citer

Toshiyuki Nishiyama, Akinobu Niozu, Christoph Bostedt, Ken Ferguson, Yuhiro Sato, et al.. Refinement for single-nanoparticle structure determination from low-quality single-shot coherent diffraction data. International Union of Crystallography journal, 2020, 7 (1), pp.10-17. ⟨10.1107/S2052252519014222⟩. ⟨cea-04115269⟩
21 Consultations
10 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More