Assessment of simplified methods for quantification of [ 18 F]-DPA-714 using 3D whole-brain TSPO immunohistochemistry in a non-human primate - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Accéder directement au contenu
Article Dans Une Revue Journal of Cerebral Blood Flow and Metabolism Année : 2019

Assessment of simplified methods for quantification of [ 18 F]-DPA-714 using 3D whole-brain TSPO immunohistochemistry in a non-human primate

Clovis Tauber

Résumé

The 18 kDa translocator protein (TSPO) is the main molecular target to image neuroinflammation by positron emission tomography (PET). However, TSPO-PET quantification is complex and none of the kinetic modelling approaches has been validated using a voxel-by-voxel comparison of TSPO-PET data with the actual TSPO levels of expression. Here, we present a single case study of binary classification of in vivo PET data to evaluate the statistical performance of different TSPO-PET quantification methods. To that end, we induced a localized and adjustable increase of TSPO levels in a non-human primate brain through a viral-vector strategy. We then performed a voxel-wise comparison of the different TSPO-PET quantification approaches providing parametric [ 18 F]-DPA-714 PET images, with co-registered in vitro three-dimensional TSPO immunohistochemistry (3D-IHC) data. A data matrix was extracted from each brain hemisphere, containing the TSPO-IHC and TSPO-PET data for each voxel position. Each voxel was then classified as false or true, positive or negative after comparison of the TSPO-PET measure to the reference 3D-IHC method. Finally, receiver operating characteristic curves (ROC) were calculated for each TSPO-PET quantification method. Our results show that standard uptake value ratios using cerebellum as a reference region (SUV CBL ) has the most optimal ROC score amongst all non-invasive approaches.

Domaines

Imagerie

Dates et versions

cea-04428124 , version 1 (31-01-2024)

Identifiants

Citer

Nadja van Camp, Yaël Balbastre, Anne-Sophie Herard, Sonia Lavisse, Clovis Tauber, et al.. Assessment of simplified methods for quantification of [ 18 F]-DPA-714 using 3D whole-brain TSPO immunohistochemistry in a non-human primate. Journal of Cerebral Blood Flow and Metabolism, 2019, 40 (5), pp.1103-1116. ⟨10.1177/0271678X19859034⟩. ⟨cea-04428124⟩
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