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Article Dans Une Revue Methods in Molecular Biology Année : 2022

From methylome to integrative analysis of tissue specificity

Résumé

DNA methylation is the most studied epigenetic mark in both plants and animals. The gold standard for assaying genome-wide DNA methylation at single-base resolution is whole-genome bisulfite sequencing (WGBS). Here, we describe an improved procedure for WGBS and original bioinformatic workflows applied to unravel tissue-specific variations of the methylome in relation to gene expression and accumulation of secondary metabolites in the medicinal plant Catharanthus roseus.
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cea-04346942 , version 1 (15-12-2023)

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Thomas Duge de Bernonville, Christian Daviaud, Cristian Chaparro, Jörge Tost, Stéphane Maury. From methylome to integrative analysis of tissue specificity. Methods in Molecular Biology, 2022, Catharanthus roseus Methods and Protocols, 2505, pp.223-240. ⟨10.1007/978-1-0716-2349-7_16⟩. ⟨cea-04346942⟩
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