Roles of the F-domain in [FeFe] hydrogenase - CEA - Commissariat à l’énergie atomique et aux énergies alternatives Access content directly
Journal Articles Biochimica biophysica acta (BBA) - Bioenergetics Year : 2018

Roles of the F-domain in [FeFe] hydrogenase


The role of accessory Fe-S clusters of the F-domain in the catalytic activity of M3-type [FeFe] hydrogenase and the contribution of each of the two Fe-S surface clusters in the intermolecular electron transfer from ferredoxin are both poorly understood. We designed, constructed, produced and spectroscopically, electrochemically and biochemically characterized three mutants of Clostridium acetobutylicum CaHydA hydrogenase with modified Fe-S clusters: two site-directed mutants, HydA_C100A and HydA_C48A missing the FS4C and the FS2 surface Fe-S clusters, respectively, and a HydA_Delta DA mutant that completely lacks the F-domain. Analysis of the mutant enzyme activities clearly demonstrated the importance of accessory clusters in retaining full enzyme activity at potentials around and higher than the equilibrium 2H(+)/H-2 potential but not at the lowest potentials, where all enzymes have a similar turnover rate. Moreover, our results, combined with molecular modelling approaches, indicated that the FS2 cluster is the main gate for electron transfer from reduced ferredoxin.

Dates and versions

cea-01950818 , version 1 (11-12-2018)



Charles Gauquelin, Carole Baffert, Pierre Richaud, Emma Kamionka, Emilien Etienne, et al.. Roles of the F-domain in [FeFe] hydrogenase. Biochimica biophysica acta (BBA) - Bioenergetics, 2018, 1859 (2), pp.69-77. ⟨10.1016/j.bbabio.2017.08.010⟩. ⟨cea-01950818⟩
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