In vivo assimilation of one-carbon via a synthetic reductive glycine pathway in escherichia coli - Génoscope-CNS Access content directly
Journal Articles ACS Synthetic Biology Year : 2018

In vivo assimilation of one-carbon via a synthetic reductive glycine pathway in escherichia coli

Madeleine Bouzon
Volker Doring
Arren Bar-Even
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Abstract

Assimilation of one-carbon compounds presents a key biochemical challenge that limits their use as sustainable feedstocks for microbial growth and production. The reductive glycine pathway is a synthetic metabolic route that could provide an optimal way for the aerobic assimilation of reduced C1 compounds. Here, we show that a rational integration of native and foreign enzymes enables the tetrahydrofolate and glycine cleavage/synthase systems to operate in the reductive direction, such that Escherichia coli satisfies all of its glycine and serine requirements from the assimilation of formate and CO$_2$. Importantly, the biosynthesis of serine from formate and CO$_2$ does not lower the growth rate, indicating high flux that is able to provide 10% of cellular carbon. Our findings assert that the reductive glycine pathway could support highly efficient aerobic assimilation of C1-feedstocks.
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cea-04313477 , version 1 (12-02-2024)

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Oren Yishai, Madeleine Bouzon, Volker Doring, Arren Bar-Even. In vivo assimilation of one-carbon via a synthetic reductive glycine pathway in escherichia coli. ACS Synthetic Biology, 2018, 7 (9), pp.2023-2028. ⟨10.1021/acssynbio.8b00131⟩. ⟨cea-04313477⟩
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