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Article Dans Une Revue Biotechnology Reports Année : 2020

Genotyping on blood and buccal cells using loop-mediated isothermal amplification in healthy humans

Résumé

Genetic variations contribute to phenotypic individual vulnerabilities to sleep debt, particularly for five single nucleotide polymorphisms (SNPs). Loop-mediated isothermal amplification and melting curve analysis (LAMP-MC) is a recently developed method to characterize SNPs. The aim of present study was to evaluate the LAMP-MC method on blood and buccal cells for detection of five SNPs of interest in healthy humans. We first analyzed signals obtained from LAMP-MC method on 42 samples. Then we compared the results with those of referent TaqMan method. The LAMP-MC method produced specific melting curves for the five SNPs. A high concordance of genotyping results was observed between the two methods for rs5751876_ADORA2A, rs1800629_TNF-α, rs73598374_ADA and rs228697_PER3 in blood and saliva (Cohen’s kappa coefficient >0.80). A good agreement ( = 0.61) was observed for rs4680_COMT in blood only. LAMP-MC is a simple and reliable method to study genetic influences on health, sleep debt-related performance impairments and countermeasures.

Dates et versions

cea-04419478 , version 1 (26-01-2024)

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Citer

Catherine Drogou, Fabien Sauvet, Mégane Erblang, Liselot Detemmerman, Céline Derbois, et al.. Genotyping on blood and buccal cells using loop-mediated isothermal amplification in healthy humans. Biotechnology Reports, 2020, 26, pp.e00468. ⟨10.1016/j.btre.2020.e00468⟩. ⟨cea-04419478⟩
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