%0 Journal Article %T Implementation of a Reductive Route of One-Carbon Assimilation in Escherichia coli through Directed Evolution %+ Génomique métabolique (UMR 8030) %+ Max Planck Institute of Molecular Physiology %A Doring, Volker %A Darii, Ekaterina %A Yishai, Oren %A Bar-Even, Arren %A Bouzon, Madeleine %Z H2020, grant agreement No 763911 %< avec comité de lecture %@ 2161-5063 %J ACS Synthetic Biology %I American Chemical Society %V 7 %N 9 %P 2029-2036 %8 2018-08-30 %D 2018 %R 10.1021/acssynbio.8b00167 %K formate assimilation %K one-carbon metabolism %K glycine cleavage complex %K directed evolution %K long-term continuous culture %K synthetic metabolism %Z Life Sciences [q-bio]/BiotechnologyJournal articles %X Endowing biotechnological platform organismswith new carbon assimilation pathways is a key challenge forindustrial biotechnology. Here we report progress toward theconstruction of formatotrophic Escherichia coli strains. Glycineand serine, universal precursors of one-carbon compoundsoxidized during heterotrophic growth, are produced fromformate and CO2 through a reductive route. An adaptiveevolution strategy was applied to optimize the enzymatic steps ofthis route in appropriate selection strains. Metabolic labelingexperiments with 13C-formate confirm the redirected carbonflow.These results demonstrate the high plasticity of the centralcarbon metabolism of E. coli and the applicative potential ofdirected evolution for implementing synthetic pathways inmicroorganisms. %G English %L cea-02299001 %U https://cea.hal.science/cea-02299001 %~ CEA %~ CNRS %~ UNIV-EVRY %~ OPENAIRE %~ CEA-UPSAY %~ GENOMIQUE-METABOLIQUE %~ UNIV-PARIS-SACLAY %~ UNIV-EVRY-SACLAY %~ CEA-UPSAY-SACLAY %~ JACOB %~ CEA-DRF %~ GENOSCOPE %~ TEST-HALCNRS %~ GS-BIOSPHERA