%0 Journal Article %T Towards a clinical application of freeze-dried squalene-based nanomedicines %+ Institut Galien Paris-Sud (IGPS) %+ Plateforme d’évaluation préclinique (PFEP) %+ Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire (LIONS) %A Rouquette, Marie %A Ser-Le Roux, Karine %A Polrot, Mélanie %A Bourgaux, Claudie %A Michel, Jean-Philippe %A Testard, Fabienne %A Gobeaux, Frédéric %A Lepetre-Mouelhi, Sinda %< avec comité de lecture %@ 1061-186X %J Journal of Drug Targeting %I Informa Healthcare %V 27 %P 1-10 %8 2019-02-21 %D 2019 %R 10.1080/1061186X.2019.1566340 %Z Chemical Sciences/Material chemistry %Z Chemical Sciences/Analytical chemistry %Z Chemical Sciences/Medicinal ChemistryJournal articles %X Squalene-adenosine (SQAd) nanoparticles (NPs) were found to display promising pharmacological activity similar to many other nanomedicines, but their long-term stability was still limited, and their preparation required specific know-how and material. These drawbacks represented important restrictions for their potential use in the clinic. Freeze-drying nanoparticles is commonly presented as a solution to allow colloidal stability, but this process needs to be adapted to each nanoformulation. Hence, we aimed at developing a specific protocol for freeze-drying SQAd NPs while preserving their structural features. NPs were lyophilised, resuspended and analysed by dynamic light scattering, atomic force microscopy and small-angle scattering. Among four different cryoprotectants, trehalose was found to be the most efficient in preserving NPs physico-chemical characteristics. Interestingly, we identified residual ethanol in NP suspensions as a key parameter which could severely affect the freeze-drying outcome, leading to NPs aggregation. Long-term stability was also assessed. No significant change in size distribution or zeta potential could be detected after three-month storage at 4?°C. Finally, freeze-dried NPs innocuity was checked in vitro on cultured hepatocytes and in vivo on mice. In conclusion, optimisation of freeze-drying conditions resulted in safe lyophilised SQAd NPs that can be easily stored, shipped and simply reconstituted into an injectable form. %G English %L cea-02068204 %U https://cea.hal.science/cea-02068204 %~ CEA %~ CNRS %~ UNIV-PSUD %~ FNCLCC %~ IGR %~ PUBNIMBE %~ DSV %~ INC-CNRS %~ IRAMIS-NIMBE %~ CEA-UPSAY %~ INSERM-SACLAY2 %~ UNIV-PARIS-SACLAY %~ UNIV-PSUD-SACLAY %~ CEA-UPSAY-SACLAY %~ IGR-SACLAY %~ CEA-DRF %~ TEST-HALCNRS %~ GS-ENGINEERING %~ IRAMIS %~ GS-CHIMIE %~ GS-PHYSIQUE %~ GS-HEALTH-DRUG-SCIENCES %~ INSTITUT-SCIENCES-LUMIERE %~ IGPS %~ TEST2-HALCNRS %~ AMMICA