%0 Journal Article %T Irradiation Effects on Polymer-Grafted Gold Nanoparticles for Cancer Therapy %+ Laboratoire Innovation en Chimie des Surfaces et NanoSciences (LICSEN UMR 3685) %+ Laboratoire Interdisciplinaire sur l'Organisation Nanométrique et Supramoléculaire (LIONS) %+ Laboratoire Léon Brillouin (LLB - UMR 12) %+ Groupe matériaux et santé %+ Transporteurs et Imagerie, Radiothérapie en Oncologie et Mécanismes biologiques des Altérations du Tissu Osseux (TIRO-MATOs - UMR E4320) %+ Immunite anti-tumorale et chimiotactisme. Adenocarcinomes et métastases %A Le Goas, Marine %A Paquirissamy, Aurélie %A Gargouri, Dorra %A Fadda, Giulia %A Testard, Fabienne %A Aymes-Chodur, C. %A Jubeli, Emile %A Pourcher, Thierry %A Cambien, Béatrice %A Palacin, Serge %A Renault, Jean-Philippe %A Carrot, Géraldine %< avec comité de lecture %@ 2576-6422 %J ACS Applied Bio Materials %I ACS Publications %V 2 %P 144-154 %8 2018-12-07 %D 2018 %R 10.1021/acsabm.8b00484 %Z Chemical Sciences/Material chemistryJournal articles %X In the context of cancer treatment, gold nanoparticles (AuNPs) are considered as very promising radiosensitizers. Here, well-defined polymer-grafted AuNPs were synthesized and studied under gamma irradiation to better understand the involved radiosensitizing mechanisms. First, various water-soluble and well-defined thiol-functionalized homopolymers and copolymers were obtained through Atom Transfer Radical Polymerization. They were then used as ligands in the one-step synthesis of AuNPs, resulting in stable hybrid metal-polymer nanoparticles. Second, these nano-objects were irradiated in solution by gamma rays at different doses. Structures were fully characterized through SEC, SAXS and SANS measurements, prior and after irradiation. We were thus able to quantify and to localize radiation impacts onto the grafted polymers, revealing the production sites of reactive species around AuNPs. Both external and near-surface scissions were observed. Interestingly, the ratio between these two effects was found to vary according to the nature of polymer ligands. Medium-range and long-distance dose enhancements could not be identified from the calculated scission yields, but several mechanisms were considered to explain high yields found for near-surface scissions. Then, cytotoxicity was shown to be equivalent for both non-irradiated and irradiated polymer-grafted NPs, suggesting that released polymer fragments were non-toxic. Finally, the potential to add bioactive molecules such as anticancer drugs has been explored by grafting doxorubicin (DOX) onto the polymer corona. This may lead to nano-objects combining both radiosensitization and chemotherapy effects. This work is the first one to study in details the impact of radiation on radiosensitizing nano-objects combining physical, chemical and biological analyses. %G English %2 https://cea.hal.science/cea-01951373/document %2 https://cea.hal.science/cea-01951373/file/LeGoas_ACSABM2019_V0.pdf %L cea-01951373 %U https://cea.hal.science/cea-01951373 %~ INSERM %~ CEA %~ UNICE %~ CNRS %~ UNIV-PSUD %~ PUBNIMBE %~ INC-CNRS %~ IRAMIS-NIMBE %~ IRAMIS-LLB %~ CEA-UPSAY %~ UNIV-PARIS-SACLAY %~ UNIV-PSUD-SACLAY %~ CEA-UPSAY-SACLAY %~ UNIV-COTEDAZUR %~ JOLIOT %~ CEA-DRF %~ SHFJ %~ CEA-CAD %~ BIAM %~ TEST-HALCNRS %~ ANR %~ GS-ENGINEERING %~ IRAMIS %~ GS-CHIMIE %~ GS-PHYSIQUE %~ INSTITUT-SCIENCES-LUMIERE %~ MAT-SANTE