%0 Journal Article %T Carboxylated nanodiamonds can be used as negative reference in in vitro nanogenotoxicity studies %+ Impact de l'environnement chimique sur la santé humaine - ULR 4483 (IMPECS) %+ Institut Pasteur de Lille %+ Servier Group %+ Laboratoire de Cancérologie Expérimentale (LCE) %+ Laboratoire Capteurs Diamant (LCD-LIST) %A Moche, Hélène %A Paget, V. %A Chevalier, D. %A Lorge, E. %A Claude, N. %A Girard, Hugues %A Arnault, Jean-Charles %A Chevillard, S. %A Nesslany, F. %Z This work was funded by the French national research program NANOTRANS, the NanoSciences and Technology pour la Sante CEA-Transverse programs, the Dim C'Nano IdF, the EU H2020 Program NANoREG 1 and NaNoREG 2 and the French Agency for Food, Environmental and Occupational Health Safety (ANSES). %< avec comité de lecture %@ 0260-437X %J Journal of Applied Toxicology %I Wiley %V 37 %N 8 %P 954-961 %8 2017-08 %D 2017 %R 10.1002/jat.3443 %M 28165139 %K BIOCOMPATIBILITY %K DIAMOND NANOPARTICLES %K Comet Assay %K CARBON NANOTUBES %K CELLS %K VIVO %K CYTOTOXICITY %K in vitro %K micronucleus assay %K FLUORESCENT NANODIAMONDS %K Nanodiamond %K genotoxicity %K CANCER %K ASSAY %K DELIVERY %K diamond %K instrumentation %K biomaterial %Z Life Sciences [q-bio]/Bioengineering %Z Life Sciences [q-bio]/Toxicology %Z Engineering Sciences [physics]/Micro and nanotechnologies/MicroelectronicsJournal articles %X Nanodiamonds (NDs) are promising nanomaterials for biomedical applications. However, a few studies highlighted an in vitro genotoxic activity for detonation NDs, which was not evidenced in one of our previous work quantifying γ-H2Ax after 20 and 100 nm high-pressure high-temperature ND exposures of several cell lines. To confirm these results, in the present work, we investigated the genotoxicity of the same 20 and 100 nm NDs and added intermediate-sized NDs of 50 nm. Conventional in vitro genotoxicity tests were used, i.e., the in vitro micronucleus and comet assays that are recommended by the French National Agency for Medicines and Health Products Safety for the toxicological evaluation of nanomedicines. In vitro micronucleus and in vitro comet assays (standard and hOGG1-modified) were therefore performed in two human cell lines, the bronchial epithelial 16HBE14o– cells and the colon carcinoma T84 cells. Our results did not show any genotoxic activity, whatever the test, the cell line or the size of carboxylated NDs. Even though these in vitro results should be confirmed in vivo, they reinforce the potential interest of carboxylated NDs for biomedical applications or even as a negative reference nanoparticle in nanotoxicology. %G English %2 https://cea.hal.science/cea-01801602/document %2 https://cea.hal.science/cea-01801602/file/article_HeleneMoche.pdf %L cea-01801602 %U https://cea.hal.science/cea-01801602 %~ CEA %~ RIIP %~ RIIP_LILLE %~ OPENAIRE %~ DRT %~ CEA-UPSAY %~ UNIV-PARIS-SACLAY %~ CEA-UPSAY-SACLAY %~ LIST %~ UNIV-LILLE %~ DM2I %~ GS-ENGINEERING %~ GS-COMPUTER-SCIENCE %~ DIN