cBAF generates subnucleosomes that expand OCT4 binding and function beyond DNA motifs at enhancers - Institut de Biologie François JACOB
Article Dans Une Revue (Data Paper) Nature Structural and Molecular Biology Année : 2024

cBAF generates subnucleosomes that expand OCT4 binding and function beyond DNA motifs at enhancers

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The canonical BRG/BRM-associated factor (cBAF) complex is essential for chromatin opening at enhancers in mammalian cells. However, the nature of the open chromatin remains unclear. Here, we show that, in addition to producing histone-free DNA, cBAF generates stable hemisome-like subnucleosomal particles containing the four core histones associated with 50–80 bp of DNA. Our genome-wide analysis indicates that cBAF makes these particles by targeting and splitting fragile nucleosomes. In mouse embryonic stem cells, these subnucleosomes become an in vivo binding substrate for the master transcription factor OCT4 independently of the presence of OCT4 DNA motifs. At enhancers, the OCT4–subnucleosome interaction increases OCT4 occupancy and amplifies the genomic interval bound by OCT4 by up to one order of magnitude compared to the region occupied on histone-free DNA. We propose that cBAF-dependent subnucleosomes orchestrate a molecular mechanism that projects OCT4 function in chromatin opening beyond its DNA motifs.
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hal-04639693 , version 1 (07-01-2025)

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Marina Nocente, Anida Mesihovic Karamitsos, Emilie Drouineau, Manon Soleil, Waad Albawardi, et al.. cBAF generates subnucleosomes that expand OCT4 binding and function beyond DNA motifs at enhancers. Nature Structural and Molecular Biology, 2024, ⟨10.1038/s41594-024-01344-0⟩. ⟨hal-04639693⟩
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