TEs as a major driver of genomic variation in trees ? Jumping MITEs in common beech revealed by TIPs and eccDNA
Résumé
Transposable elements (TEs) are predominant in most plant genomes but their impact and activity has been mostly studied in annual species. Beech trees (Fagus sylvatica) make up most of European forests and are highly threatened by drought and climate change. We sought the investigation of the genomic diversity caused by TEs and their adaptive potential in this long-living species. We aimed at characterizing Transposable Element Insertion Polymorphisms (TIPs) playing key roles in genome evolution by analysing TIPs in a single population of 147 common beech trees, using 672 Long Terminal Repeats Retrotransposons (LTR) and 558 Miniature Inverted Transposable Elements (MITEs) families. We further analysed somatic structural variants (SV) generated by TEs. Given the high level of very recent TE activity in common beech, we investigated ongoing TE mobility using extrachromosomal circular DNA (eccDNA) sequencing or mobilome-seq, eccDNA being a useful proxy for identifying active TEs.
Domaines
Sciences du Vivant [q-bio]Origine | Fichiers produits par l'(les) auteur(s) |
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