RSL24D1 sustains steady-state ribosome biogenesis and pluripotency translational programs in embryonic stem cells - CRCL-Reprogrammation cellulaire, cellules souches et oncogenèse Access content directly
Journal Articles Nature Communications Year : 2023

RSL24D1 sustains steady-state ribosome biogenesis and pluripotency translational programs in embryonic stem cells

Bigitha Bennychen
  • Function : Author
Annie Adrait
  • Function : Author
Yohann Couté
Mathieu Gabut

Abstract

Embryonic stem cell (ESC) fate decisions are regulated by a complex circuitry that coordinates gene expression at multiple levels from chromatin to mRNA processing. Recently, ribosome biogenesis and translation have emerged as key pathways that efficiently control stem cell homeostasis, yet the underlying molecular mechanisms remain largely unknown. Here, we identified RSL24D1 as highly expressed in both mouse and human pluripotent stem cells. RSL24D1 is associated with nuclear pre-ribosomes and is required for the biogenesis of 60S subunits in mouse ESCs. Interestingly, RSL24D1 depletion significantly impairs global translation, particularly of key pluripotency factors and of components from the Polycomb Repressive Complex 2 (PRC2). While having a moderate impact on differentiation, RSL24D1 depletion significantly alters ESC self-renewal and lineage commitment choices. Altogether, these results demonstrate that RSL24D1-dependant ribosome biogenesis is both required to sustain the expression of pluripotent transcriptional programs and to silence PRC2-regulated developmental programs, which concertedly dictate ESC homeostasis.
Fichier principal
Vignette du fichier
41467_2023_Article_36037.pdf (2.94 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-04010880 , version 1 (25-05-2023)

Identifiers

Cite

Sébastien Durand, Marion Bruelle, Fleur Bourdelais, Bigitha Bennychen, Juliana Blin-Gonthier, et al.. RSL24D1 sustains steady-state ribosome biogenesis and pluripotency translational programs in embryonic stem cells. Nature Communications, 2023, 14 (1), pp.356. ⟨10.1038/s41467-023-36037-7⟩. ⟨hal-04010880⟩
69 View
57 Download

Altmetric

Share

Gmail Facebook X LinkedIn More